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PROCEDURE FOR OBTAINING
DIALYSATE CULTURE AND CELL COUNT
PURPOSE:
To obtain a sample of effluent for diagnostic
testing using sterile technique
EQUIPMENT:
- Mask
- Two 10ml syringes
- One 20ml syringe
- Three 20 gauge 1" needles
- Sterile culture container
- One 3.5ml purple top tube
- Three patient identification labels
- Exam gloves (non sterile)
- Two blood culture bottles
- Three alcohol pads
NURSING ACTION:
PROCEDURE STEPS:
A. To obtain dialysate
sample from CAPD patient:
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Have patient do an
exchange using their usual procedure.
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Take spent bag of
dialysate (no longer connected to patient in any way) and invert to
drain back into fill bag (fill bag has an injection port).
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Mask.
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Wash hands, put on
gloves.
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Place a needle on
each syringe.
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Prepare medication
port of dialysate bag or by doing a 10-second circular scrub with an
alcohol pad.
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Puncture prepared
area with sterile syringe/needle and draw up dialysate.
KEYPOINT: Specimen requirements:
For routine culture:
Two 10ml syringes of dialysate are needed,
one for each blood culture bottle.
For cell count:
3.5ml of dialysate in a purple top tube and 5ml in a red top tube
without separator is needed.
For AFB or fungal cultures and/or gram stain:
10ml of dialysate in a sterile culture
container is needed.
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Disinfect top of
purple top tube prior to injecting dialysate by performing a
10-second circular scrub with an alcohol pad.
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Inject 3.5ml of
dialysate into purple top tube for cell count.
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Inject 10ml of
dialysate into each blood culture bottle.
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Inject 10ml of
dialysate into the sterile culture container for AFB, fungus.
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Inject 5ml of dialysate
into red top tube without separator for Gram Stain.
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Label all specimens as
Dialysate for specific test.
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Send all specimens to
laboratory.
REFERENCES:
American Nephrology
Nursing Association
ANNA: Core Curriculum for Nephrology Nursing, 2000.
National Institutes of Health, NIDDK
Laboratory Guide
RESOURCE PERSON(S):
Cathy Brothers, RN, Valerie
Bosley, RN
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