Project 5. Mitochondrial Respiration, Oxidative Damage, & Aging
Rationale and Aims
Project 5 examines yeast mitochondrial respiratory functions, bioenergetics, and damage in order to test
whether respiratory control determines generation of reactive oxygen species and yeast longevity, and could be attenuated
by transgenic expression of alternative oxidase or uncoupling protein, in yeast or in metazoan species. We will determine
metabolic profiles under conditions extending or shortening chronological life span, and will further explore mutation levels of
mitochondrial DNA as a measure of accrued mitochondrial damage and as a potential mode of intervention.
We are using yeast as a model system for assessing interventions that will modify the metabolic production
of reactive oxygen species. We find that these interventions do have effects on longevity of yeast in culture,
and have begun transferring this genetic/metabolic engineering to our metazoan models, C. elegans
Slide Presentation of Recent Data
Joan E. McEwen, Ph.D., Project Leader
Magomed Khaidakov, M.D., Ph.D.
Joseph Hof, B.A.
Ania M. Galuszka, B.Sc.
Clayton Johnson, M.Sc.