Core B

The Metabolic Assessment Core

John J. Thaden, Ph.D.

Research Assistant Professor

John Thaden received his Sc.B. in biomedical sciences from Brown University in Providence RI, and, after employment as an industrial pure water chemist and a radiobiology research technician, his Ph.D. in biochemistry from the Johns Hopkins University in Baltimore MD. A National Institute on Aging National Research Service Award partially funded postdoctoral studies with Dr. Robert Reis at the University of Arkansas. Subsequently, Dr. Thaden was granted a College of Medicine pilot award and joined their faculty as an instructor. He was promoted to research assistant professor of geriatrics in 2000, and in 2003, began service both as the leader of the Metabolic Assessment Core component of the 5-year, $5 million National Institute on Aging program project described in these pages, and as director of the UAMS Small Molecule Mass Spectrometry resource core.

Dr. Thaden is a respected member of the international community of Caenorhabditis elegans nematode researchers. He is affiliated also with the Metabolomics Society, the American Chemical Society, the American Society for Mass Spectrometry, the International Society of Lyophilization and the Society for Free Radical Biology and Medicine.

Dr. Thaden's research interests center on changes in metabolism and gene expression that retard the rate of organismal aging, and on methods from biochemistry, analytical chemistry and multivariate statistics that permit such changes to be detected, quantified and evaluated for significance. He has particular interest in global survey tools (metabolomics, genomics), gas chromatography, high performance liquid chromatography, amperometric detection, electrospray ionization and quadrupole mass spectrometry.

Publications:

1. Redpath JL, Thaden JJ, Fong RL (1986) Mutation induction in Escherichia coli WP2 uvrA by Cerenkov emission associated with ¹³⁷Cs gamma irradiation. Radiation Res. 105: 8-17.

2. Thaden J, Miller PS (1993) Photoaffinity behavior of a conjugate of oligonucleoside methylphosphonate, rhodamine, and psoralen in the presence of complementary oligonucleotides. Bioconjugate Chem. 4: 386-394.

3. Thaden J, Miller PS (1993) Automated synthesis of oligodeoxyribonucleoside methylphosphonates having [N-(3-aminoprop-1-yl)-2-aminoethyl] phosphate or methylphosphonic acid at the 3’ end using a modified controlled pore glass support. Bioconjugate Chem. 4: 395-401.

4. Beneš H, Shelton RS, Weinstein RS, Zheng W, Thaden JJ, Jilka RL, Manolagas SC, Shmookler Reis RJ (2000) Chromosomal mapping of osteopenia-associated quantitative trait loci using closely related mouse strains. J. Bone Min. Res. 15: 26-33.

5. Ayyadevara S, Thaden JJ, Shmookler Reis RJ (2000) Anchor polymerase chain reaction display: A high-throughput method to resolve, score and isolate dimorphic genetic markers based on interspersed repetitive DNA elements. Analyt. Biochem. 284: 19-28.

6. Ayyadevara S, Thaden JJ, Shmookler Reis RJ (2000) Discrimination of primer 3'-nucleotide mismatch by Taq DNA polymerase during polymerase chain reaction. Analyt. Biochem. 284: 1-18.

7. Thaden JJ, Shmookler Reis RJ (2000) Ammonia, respiration, and longevity in nematodes: insights on metabolic regulation of life span from temporal rescaling. Age 23: 75-84.

8. Ayyadevara S, Ayyadevara R, Hou S, Thaden JJ, Shmookler Reis RJ (2001) Genetic mapping of quantitative trait loci governing longevity of Caenorhabditis elegans, in recombinant-inbred progeny of a Bergerac BO × RC301 interstrain cross. Genetics 157: 655-666.

9. Ayyadevara S, Ayyadevara R, Vertino A, Galecki A, Thaden JJ, Shmookler Reis RJ (2003) Genetic loci affecting fitness and life span in Caenorhabditis elegans: Categorical trait interval mapping in CL2a × Bergerac BO recombinant-inbred worms. Genetics 163: 557-570.

10. Ayyadevara S, Ayyadevara R, Vertino A, Galecki A, Thaden JJ, Shmookler Reis RJ (2003) Genetic loci affecting fitness and life span in Caenorhabditis elegans: Categorical trait interval mapping in CL2a × Bergerac BO recombinant-inbred worms. Genetics 163: 557-570.

Email: JThaden@uams.edu