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Project 5 Mitochondrial respiration, oxidative damage, and aging
Joan E. McEwen, Ph.D Associate Professor Department of Geriatrics
Research interests:
CURRICULUM VITAE
Name Joan E. McEwen
Education
1974 B.A. Tufts University, Biology, summa cum laude 1980 Ph.D. Albert Einstein College of Medicine, Molecular Biology
Professional Experience
1974-1980 Predoctoral Fellow, Albert Einstein College of Medicine Preceptor: Dr. Philip M. Silverman 1980-1984 Postdoctoral Fellow, University of Colorado Preceptor: Dr. Robert O. Poyton 1984-1993 Assistant Professor, Department of Microbiology and Molecular Genetics, UCLA 1993-1995 Associate Research Scientist, Dept. of Molecular Genetics, Beckman Research Institute, City of Hope Medical Center 1995-present Associate Professor, Dept. of Geriatrics, University of Arkansas for Medical Sciences; and Research Health Scientist, GRECC, J.L. McClellan VA Hospital
Honors and Awards
1974 Phi Beta Kappa 1975-1980 NIH Fellowship, Pre-doctoral Training Grant 1980-1981 Damon Runyan Postdoctoral Fellowship 1981-1984 NIH Postdoctoral Fellowship
Memberships
American Society for Microbiology American Association for the Advancement of Science Genetics Society of America
Research Interests Role of mitochondrial respiration and oxidative damage in organismal longevity. Nuclear-mitochondrial interactions in the development and function of mitochondria in S. cerevisiae and in the human pathogenic fungus H. capsulatum. Scholarly Service
1989 Ad hoc member of Microbiology and Virology study section for American Cancer Society
1984-1993 Ad hoc reviewer of grant proposals to Jonsson Cancer Center of UCLA
1994 Ad hoc member of Physiological Chemistry study section, NIH
2000 panelist, microbial genetics review panel, NSF
1984-present Ad hoc reviewer of manuscripts for Science, J. Bacteriol., Nucl. Acids Research, Mol. Microbiol.
Selected University or Departmental Service
1986-1990 Five Faculty search committees for the Dept. of Microbiology and Molecular Genetics at UCLA
1986-1991 Committee for design of new Microbiology and Molecular Genetics building at UCLA
1991-1993 Committee for design of renovated teaching lab space for the Dept. of Microbiology and Molecular Genetics at UCLA
1989-1992 Graduate Admissions Committee for the Molecular Biology Institute at UCLA
1988-1993 Chairman of Honors program for majors in the Department of Microbiology and Molecular Genetics at UCLA
1984-1995 Mentor for four Ph.D. students who conducted their dissertation research in my laboratory at UCLA. Student names: Jeffrey D. Ohmen, Maria L. Valencik, Kathleen M. Calder, and Glenn M. Manthey.
1994-1995 Graduate Admissions Committee for the Ph.D. program at Beckman Research Institute, City of Hope Medical Center
1999-2002 Vice chairman of the Animal Care and Use Committee of Central Arkansas Veterans Healthcare System.
2002-present Chairman of the Animal Care and Use Committee of Central Arkansas Veterans Healthcare System
1999-2002 Member of Research and Development committee of Central Arkansas Veterans Healthcare system
Selected community service
1989, 1990 Faculty mentor in minority undergraduate summer research program at UCLA
1998-2001 Membership chairman and member of board of Directors of Diamond Edge Figure Skating Club of Little Rock, AR (a 501C3 tax exempt organization with charitable activities)
2004-present Member of board of Directors of Diamond Edge Figure Skating Club of Little Rock, AR (a 501C3 tax exempt organization with charitable activities)
Selected Teaching Experience
Microbiology and Molecular Genetics 101, UCLA This is an undergraduate upper division course on "Fundamentals of Microbiology". This course, which consists of both lecture and laboratory, is required for majors of two departments - Dept. of Microbiology and Molecular Genetics and Dept. of Biochemistry - and is an important elective course for Biology majors. Times taught: 1986, Spring 1987, Spring 1988, Spring 1989, Spring 1989, Fall 1991, Spring 1991, Fall 1992, Fall
Microbiology and Molecular Genetics 195H, UCLA This is an upper division seminar/discussion course for students in the Honors program. Times taught: 1987, Spring 1989, Spring 1990, Spring 1991, Spring 1992, Spring 1993, Spring
Graduate seminar courses at UCLA, 1984-1995, on a variety of topics, including "Mitochondrial gene expression"; "Regulation of prokaryotic metabolism"; "Regulation of cytochrome genes in bacteria and yeast"; "Mechanisms of RNA splicing"; "Translational control of gene expression"; "Protein targeting in prokaryotes and eukaryotes"; "Prokaryotic development and cell-cell interactions".
Mentoring of undergraduate students in independent research, 1984-1995. I had from 1-3 undergraduate students per quarter working in my laboratory on independent research projects, for which they received course credit.
Lectures on protein synthesis to graduate students at Beckman Research Institute at City of Hope Medical Center Times taught: 1994 1995
Lectures (4) on yeast genetics to graduate students in MBIM 5123 class, Dept. of Microbiology and Immunology, UAMS Times taught: 1996 1997 1998
Lectures (6) on bacterial and yeast genetics to graduate students in MBIM 5023 class (Principles of Microbiology, Dept. of Microbiology and Immunology, UAMS Times taught: 1998 1999 2000
Lectures (3) on yeast physiology and metabolism to graduate students in MBIM 5023 class (Principles of Microbiology) Times taught: 2002 2003 2004 2005 Lectures (2) on fungal pathogens in Medical Microbiology for Medical Students class Times taught: 1999 2000 2001 2002 2003 2004
Lectures (2) on prokaryotic protein synthesis to graduate students in Physiology/MBIM Gene expression course Times taught: 2001
BIBLIOGRAPHY
Published articles (peer reviewed)
l. McEwen, J. and P. Silverman. l980. Chromosomal mutations of Escherichia coli that alter expression of conjugative plasmid functions. Proc. Natl. Acad. Sci. U.S.A. 77:513-517.
2. Silverman, P., K. Nat, J. McEwen, and R. Birchman. l980. Selection of Escherichia coli K12 chromosomal mutants that prevent expression of F-plasmid functions. J. Bacteriol. 143:1519-1523.
3. McEwen, J., and P. Silverman. l980. Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: Identification of genes cpxA and cpxB. J. Bacteriol. l44:60-67.
4. McEwen, J., and P. Silverman. l980. Mutations in genes cpxA and cpxB of Escherichia coli K-12 cause a defect in isoleucine and valine syntheses. J. Bacteriol. l44:68-73.
5. Sutton, A., T. Newman, J. McEwen, P. Silverman and M. Freundlich. l982. Mutations in genes cpxA and cpxB of Escherichia coli K12 cause a defect in Acetohydroxyacid Synthase I function in vivo. J. Bacteriol. l5l:976-982.
6. McEwen, J. and P. Silverman. l982. Mutations in genes cpxA and cpxB alter the protein composition of Escherichia coli inner and outer membranes. J. Bacteriol. l5l:l553-l559.
7. McEwen, J., L. Sambucetti, and P. Silverman. l983. Synthesis of outer membrane proteins in cpxA cpxB mutants of Escherichia coli K12. J. Bacteriol. l54:375-382.
8. Cumsky, M., J. McEwen, C. Ko, and R. Poyton. l983. Nuclear genes for mitochondrial proteins: Identification and isolation of a structural gene for subunit V of Yeast cytochrome c oxidase. J. Biol. Chem. 258:13418-13421.
9. McKee, E., J. McEwen, and R. Poyton. 1984. Mitochondrial gene expression in Saccharomyces cerevisiae. II. Fidelity of translation in isolated mitochondria from wild-type and respiratory-deficient mutant cells. J. Biol. Chem. 259:9332-9338.
10. McEwen, J., M. Cumsky, C. Ko, S. Power and R. Poyton. l984. Mitochondrial membrane biogenesis: Characterization and use of Pet mutants to clone the nuclear gene coding for subunit V of Yeast cytochrome c oxidase. J. Cell. Bioch. 24:229-242.
11. McEwen, J., V. Cameron and R. Poyton. l985. A rapid method for the isolation and screening of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae. J. Bacteriol. 161:831-835.
12. McEwen, J., C. Ko,
B. Kloeckener-Gruissem and R. O. Poyton.
1986. Nuclear functions required
for cytochrome c oxidase biogenesis in Saccharomyces cerevisiae. Characterization of mutants in 34
complementation groups. J. Biol.
Chem. 261:11872-11879.
14. Kloeckener-Gruissem, B., J. McEwen and R. Poyton. 1988. Identification of a third nuclear protein-coding gene required specifically for expression of the mitochondrial COX3 gene in S. cerevisiae. J. Bacteriol. 170: 1399-1402.
15. Ohmen, J., B. Kloeckener-Gruissem, and J. McEwen. 1988. Molecular cloning and nucleotide sequence of the nuclear PET122 gene that is required for expression of the mitochondrial COX3 gene in S. cerevisiae. Nucleic Acids Res. 16: 10783-10802.
16. Valencik, M.L, B. Kloeckener-Gruissem, R.O. Poyton, and J.E. McEwen. 1989. Disruption of the yeast nuclear PET54 gene blocks excision of mitochondrial intron aI5ß from pre-mRNA for cytochrome c oxidase subunit I. EMBO J. 8: 3899-3904.
17. Calder, K.M., and J.E. McEwen. 1990. Nucleotide sequence of the gene encoding cytochrome c oxidase subunit VII from Saccharomyces cerevisiae. Nucleic Acids Res. 18: 1632-1632.
18. Ohmen, J.D., K.A. Burke, and J.E. McEwen. 1990. Divergent overlapping transcripts at the PET122 locus in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 3027-3035.
19. Valencik, M.L., and J.E. McEwen. 1991. Genetic evidence that different functional domains of the PET54 gene product facilitate expression of the mitochondrial genes COX1 and COX3 in S. cerevisiae. Mol. Cell. Biol. 11: 2399-2405.
20. Calder, K.M., and J.E. McEwen. 1991. Deletion of the COX7 gene in Saccharomyces cerevisiae reveals a role for cytochrome c oxidase subunit VII in assembly of remaining subunits. Mol. Micro. 5: 1769-1777.
21. Burke, K.A., and J.E. McEwen. 1991. Characterization of mRNAs and coding potential of the PET54 gene from Saccharomyces cerevisiae. Biochem. International, 25: 339-348.
22. McEwen, J.E., Hong, K.H., Preciado, G.T., and Park, S. 1993. Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae. Curr. Genet. 23: 9-14.
23. Marathe, S., and McEwen, J.E. 1995. Vectors with the GUS gene as a reporter for identifying and quantitating promoter regions in Saccharomyces cerevisiae. Gene 154: 105-107.
24. Manthey, G.M., and McEwen, J.E. 1995. The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus in Saccharomyces cerevisiae. EMBO J 14: 4031-4043.
25. Poyton, R.O., and McEwen, J.E. 1996. Cross-talk between nuclear and mitochondrial genomes. Ann. Rev. Biochem. 65: 563-607. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8811190&query_hl=1
26. Johnson, C.H., and McEwen, J.E. 1997. Mitochondrial protein synthesis is not required for efficient excision of intron aI5b from COX1 pre-mRNA in Saccharomyces cerevisiae. Mol. Gen. Genet. 256: 88-91. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9341683&query_hl=1
27. Manthey, G.M., Przybyla-Zawislak, B.D., and McEwen, J.E. 1998. The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane. Eur. J. Bioch. 255: 156-161. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9692914&query_hl=1
28. Johnson, C.H., and McEwen, J.E. 1999. Isolation of a Histoplasma capsulatum cDNA that complements a mitochondrial NAD(+)-isocitrate dehydrogenase subunit I-deficient mutant of Saccharomyces cerevisiae. Yeast 15: 799-804. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10398348&query_hl=1
29.Marathe, S.V., and McEwen, J.E. 1999. Expression of the divergent transcription unit containing the yeast PET122 and OXA1 genes. Bioch. And Mol. Biol. Int. 47: 971-977. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10410243&query_hl=1
30. Johnson, C.H., Klotz, M.G., York, J.L., Kruft, V. and McEwen, J.E. 2002. Redundancy, phylogeny and differential expression of Histoplasma capsulatum catalases. Microbiology148: 1129-1142. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11932457&query_hl=1
31. Ranganathan, G., Phan, D., Pokrovskaya, I.D., McEwen, J.E., Li, C. and Kern, P.A. 2002. The translational regulation of Liproprotein lipase by Epinephrine involves an RNA binding complex Including the Catalytic Subunit of Protein Kinase A. J. Biol. Chem. 277: 43281-43287. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12218046&query_hl=1
32. Johnson, C.H., Prigge, J.T., Warren, A.D. and McEwen, J.E. 2003. Characterization of an alternative oxidase activity of Histoplasma capsulatum. Yeast 20: 381-388. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12673621&query_hl=1
33. Missal, T.A., Lodge, J.K., and McEwen, J.E. 2004. Mechanisms of resistance to oxidative and notrosative stress: Implications for fungal survival in mammalian hosts. Eukaryotic Cell 3: 835-846. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15302816&query_hl=1
34. McEwen, J.E., Zimniak, P., Mehta, J.L., and Reis, R.J. 2005. Molecular pathology of aging and its implications for senescent coronary atherosclerosis. Current Opinion in Cardiology 20: 399-405. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16093759&query_hl=1
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