David Cue

Research Associate Professor

Ph.D: University of Iowa

Postdoctoral: University of Minnesota

Phone: (501) 686-8763
Fax: (501) 686-5359     

E-mail

Research Description:

Staphylococcus aureus is a major cause of both nosocomial and community acquired infections ranging from superficial skin infections to highly invasive and potentially lethal infections. Some S. aureus infections such as endocarditis, osteomyelitis and infections associated with indwelling medical devices, depend on the bacterium's capacity to form biofilms. Biofilms are communities of microorganisms embedded in an extracellular matrix attached to a solid surface. Biofilms are believed to afford pathogens protection against antibiotics and the host immune response. Accordingly, biofilm associated infections are often recalcitrant to treatment.

Biofilm formation is a complex, multistep process involving the regulated expression of well over 100 S. aureus genes. Our laboratory is interested in regulatory genes that govern biofilm formation. As part of this effort we are studying the how one regulatory factor, rbf for regulation of biofilm, exerts control over biofilm formation. Greater understanding of the mechanisms governing biofilm production may lead to novel therapies for treatment of staphylococcal disease.

References

Wei, L., V. Pandiripally, E. Gregory, M. Vance and D. Cue . 2005. Impact of the SpeB protease on binding of the complement regulatory proteins, factor H and factor H-like protein 1, by Streptococcus pyogenes Infect. Immun. 73(4):2040-2050.

Pandiripally, L. Wei, C. Skerka, P.F. Zipfel and D. Cue . 2003. Recruitment of Complement Factor H-like Protein 1 Promotes Intracellular Invasion by Group A Streptococci. Infect. Immun. 71:7119-7128.

Pandiripally, V., E. Gregory and D. Cue . 2002. Acquisition of Regulators of Complement Activation by Streptococcus pyogenes Serotype M1 . Infect. Immun. 70:6206-6214.

Cue, D. , H. Lam and P. P. Cleary. 2001. Genetic dissection of the Streptococcus pyogenes M1 protein: regions involved in fibronectin binding, autoaggregation and intracellular invasion. Microbio. Pathog. 31:231-242.

Cue, D . and M. Feiss. 2001. Bacteriophage lambda DNA packaging: DNA site requirements for termination and processivity . J. Mol. Biol. 311:233-240.

Cue, D ., S. O. Southern, P. J. Southern, J. Prabhakar, W. Lorelli, J. M. Smallheer, S. A. Mousa and P. P. Cleary. 2000. A nonpeptide integrin antagonist can inhibit epithelial cell ingestion of Streptococcus pyogenes by blocking formation of integrin a 5 b 1-fibronectin-M1 protein complexes . Proc. Natl. Acad. USA 97:2858-2863.

Cue, D ., P. E. Dombek, H. Lam and P. P. Cleary. 1998. Serotype M1 Streptococcus pyogenes Encodes Multiple Pathways for Entry into Human Epithelial Cells. Infect. Immun. 66:4593-4601.

Cue, D . and Feiss, M. 1998. Termination of packaging of the bacteriophage lambda chromosome: The role of cosQ in nicking at cosN . J. Mol. Biol. 280:11-29.

Cue, D . and M. Feiss. 1997. Genetic Evidence that cosQ , the Signal for Termination of Phage lambda DNA packaging, Depends on the Extent of Head Filling. Genetics. 147:7-17.

Cue, D. , H. Lam, R. L. Dillingham, R. S. Hanson and M. C. Flickinger. 1997. Genetic Manipulation of Bacillus methanolicus , a Gram-Positive, Thermotolerant Methylotroph. Appl. Environ. Microbiol. 63:1406 - 1420.

Cue, D. , Lam, H. Hanson, R. S. and Flickinger, M. 1996. Characterization of a Restriction-Modification System of the Thermotolerant Methylotroph Bacillus methanolicus . Appl. Environ. Microbiol. 62:1107-1111.

Cue, D. and M. Feiss,. 1993. The Role of cosB , the binding site for terminase, the DNA Packaging Enzyme of Bacteriophage lambda, in the Nicking Reaction. J. of Mol. Biol. 234:594-609.

Cue, D. and M. Feiss. 1993. A site Required for Termination of Packaging of the Phage lambda Chromosome. Proc. Natl. Acad. Sci. USA. 90:9290-9294.

Cue, D. and M. Feiss. 1992. Genetic Analysis of cosB , the Binding Site for Terminase, the DNA Packaging Enzyme of Bacteriophage lambda. J. of Mol. Biol. 228:58-71.

Cue, D. and M. Feiss. 1992. Genetic Analysis of Mutations Affecting Terminase, the Bacteriophage lambda DNA Packaging Enzyme, that Suppress Mutations in cosB , the Terminase Binding Site. J. of Mol. Biol. 228:72-87.

Xu, S-H. and D. Cue. 1990. Increased efficiency of oligonucleotide mutagenesis by removal of endogenous primers from preparations of M13 ssDNA. Biotechniques. 8:168-169.

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