Flourochome Chart - Dept. of Microbiology and Immunology - University of Arkansas for Medical Sciences

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What is Flow Cytometry

Protocols and Reagents

Sample Preparation

	Appointments
	FACSCalibur
	FACSAria
	Bioplex

Fluorochromes and Dyes

Fluorochrome/Dye	FACSCalibur	FACSAria
Alexa 405	N/A	407/PMT-B
Alexa 407	N/A	407/PMT-B
Alexa 430	N/A	407/PMT-A
Alexa 488	FL-1	488/PMT-E
Alexa 633	FL-4	633/PMT-B
Alexa 647	FL-4	633/PMT-B
Alexa 660	N/A	633/PMT-B
Alexa 610-PE	N/A	488/PMT-C
APC	FL-4	633/PMT-B
APC-Cy7	N/A	633/PMT-A
Cy3	FL-2	488/PMT-D
Cy5	FL-4	633/PMT-B
FITC	FL-1	488/PMT-E
Pacific Blue	N/A	407/PMT-B
PE	FL-2	488/PMT-D
PE-Cy5	FL-3	488/PMT-B
PE-Cy5.5	FL-3	488/PMT-B
PE-Cy7	FL-3	488/PMT-A
PE-Texas Red	FL-2	488/PMT-C
PerCP	FL-3	488/PMT-B
PerCP-Cy5.5	FL-3	488/PMT-B
Rhodamine 123	FL-1	488/PMT-E

Nucleic Acid Dyes

Dye	FACSCalibur	FACSAria
7-AAD	FL-3	488/PMT-B
Acridine Orange (DNA only)	FL-3	488/PMT-B

Acridine Orange (RNA only)	FL-1	488/PMT-E
DAPI	N/A	407/PMT-B
Hoechst 33342	N/A	407/PMT-B
Propidium Iodide	FL-2(Hi)/FL-3(Lo)	488/PMT-C
Pyronin Y	FL-2	488/PMT-D
Sytox Green	FL-1	488/PMT-E
Thiazole Orange	FL-1	488/PMT-E
TO-PRO 1	FL-1	488/PMT-E
TO-PRO 3	N/A	633/PMT-B

Cell Function

Fluorochrome	FACSCalibur	FACSAria
DiOCn	FL-1	488/PMT-E
Fluo-3	FL-1	488/PMT-E
Rhodamine 123	FL-1	488/PMT-E
SNARF (los pH)	FL-2	488/PMT-D
SNARF (high pH)	FL-3	488/PMT-B

Fluorescent Proteins

Protein	FACSCalibur	FACSAria
DsRED	FL-2	488/PMT-D
ECFP	N/A	407/PMT-B
EGFP	FL-1	488/PMT-E
EYFP	FL-1	488/PMT-E

**If you cannot find the fluorochrome or dye that you want to use on this page, check the equipment pages for the FACSCalibur and FACSAria . Use the excitation and emission spectra of your fluorochrome or dye to determine whether we can detect it. You may also try using the BD Biosciences Fluorescence Spectrum Viewer . **

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