2 grams paraformaldehyde / 100 ml of 1X PBS
Heat at 55 degrees for 1 hour or microwave for 10 – 30 seconds until it goes into solution.
Place on ice until use.
To fix samples: Centerfuge samples, remove supernatants, vortex pellet, resuspend cells in 0.5 ml of 2% paraformaldehyde/sample. Store samples on ice and discard unused portion of paraformaldehyde.
Note: 2% paraformaldehyde should be made fresh for each staining assay use.