This Core has a confocal microscope and morphometric analysis software,
along with multizoom, and fluorescence microscopy capability, and a
fully equipped and staffed histology laboratory for histochemical and
of PPN neurons. A. Two patch clamped PPN cells found to be
electrically coupled using electrophysiological criteria.
Reconstruction using a confocal microscope revealed several
potential points of contact. Calibration bar 40
mm. B. Patch clamped PPN
cell (magenta) immunocytochemically labeled for neurobiotin from
the recording pipette that was found to have retrobeads
(greenish white beads) previously injected into the intralaminar
thalamus, indicating that the recorded neuron was a thalamic
projection PPN neuron. The same cell was also
immunocytochemically labeled as cholinergic (red). Calibration
bar 50 mm.