Research Facilities
DNA Sequencing Facility
 The DNA Sequencing Facility uses a 3100 Genetic Analyzer (capillary technology) to provide DNA sequencing for plasmids and PCR products. A Qiagen cabinet is also present in the lab to provide select DNA and RNA isolation kits.
Contact: Allen Gies, 501-686-6927,
giesallenj@uams.edu
Proteomics Facility
 The
Proteomics Facility provides mass spectrometric analysis and identification of proteins.
It is equipped with a Thermo LTQ XL linear ion trap mass spectrometer with electron transfer dissociation capability, a PerkinElmer SCIEX MALDI-prOTOF mass spectrometer, a Waters Q-Tof Micro tandem mass spectrometer and a Thermo LTQ linear ion trap mass spectrometer. Additional gel band analysis equipment includes a ProPic imaging and spot-picking robot from Genomic Solutions, a ProGest in-gel enzymatic digestion robot from Genomic Solutions, and a Bio-Rad 2D gel electrophoresis system. Contact:
Dr. Sam Mackintosh, 501-526-5978,
mackintoshsamuelg@uams.edu
Transgenic
Facility
 The
transgenic facility generates transgenic mice via pronuclear microinjection
of DNA constructs supplied by users. Microinjections are performed in
embryos obtained from C57BL/6 or CB6F1 (a cross between BALB/c and C57BL/6) mice.
Personnel are also available for consultation regarding DNA construct design and animal husbandry. Contact: Charles O’Brien, 501-686-5607,
caobrien@uams.edu , or Joseph Goellner, 501-940-7264,
jjgoellner@uams.edu.
Microbiology & Immunology Flow Cytometry
 The Microbiology & Immunology Flow Cytometry Facilities provide researchers with an immensely powerful and diverse tool to measure multiple physical characteristics of individual cells in suspension. The flow cytometer can indicate relative cell size and density or complexity by measuring forward- and side-scattered laser light, respectively. In addition, the flow cytometer can measure relative fluorescence from fluorescent probes which bind to specific cell-associated molecules.
Contact: Andrea Fox, 501-686-5145,
afox@uams.edu
Bioluminescent and Fluorescent Imaging
Facilities
 The
Xenogen IVIS Imaging System is a highly sensitive CCD camera optimized for
biophotonic imaging (e.g. in vitro and in vivo assays using reporter cells
tagged for bioluminescence and/or fluorescence).
Contact:
Bill Hogue, 501-686-8911,
bhogue@uams.edu
Protein Biomarker
Identification
Facility
 A facility for protein biomarker
identification using SELDI (surface-enhanced laser disorption/ionization).
The SELDI is utilized primarily for clinical biomarker discovery,
identification and validation.
Contact: Larry J. Suva, 501-526-6110,
suvalarryj@uams.edu
Skeletal Phenotyping
Facility
 High resolution skeletal
imaging and analysis utilizing bone densitometry (DXA), perpipheral
quantitated computed tomography (pQCT) or micro CT (uCT). Skeletons can be
analyzed in vivo or ex vivo. When combined with histological evaluation,
detailed insight into skeletal phenotype is obtained.
Contact:
Larry J. Suva, 501-526-6110,
suvalarryj@uams.edu
Digital and Confocal Microscopy Laboratory
 Operates
and maintains computer controlled microscope workstations capable of static
and time lapse (e.g. minutes to days) imaging of living cells. Fluorescence
imaging and multicolor images from fixed and living samples are generated.
Microscopes are inverted and designed for analysis of living cells. However,
fixed samples may be studied as well.
Contact:
Richard Kurten, 501-686-8269,
kurtenrichardc@uams.edu
Radiological
Imaging Facility
 The
MicroPET Facility consists of a Siemens FOCUS MicroPET that can produce
whole body or regional PET images of animals ranging in size from mice to
small dogs; and can image the head and appendages of human subjects. An
on-site cyclotron and a fully staffed Radiochemistry Laborotory provide a
wide range of PET markers for research projects involving PET imaging.
Custom PET Markers can also be developed in collaboration with the
facility's staff.
Contact: Director: Michael J. Borrelli,
Ph.D. 501-526-5897,
mjborelli@uams.edu Manager:
Terri L. Alpe, B.S. 501-590-0457,
alpeterril@uams.edu
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