Research Facilities
Biotelemetry Facility
 Biotelemetry
offers continuous monitoring of cardiovascular parameters (e.g. blood
pressure, heart rate, electrocardiogram) in rodents. This involves surgical
implantation of transmitters (service) and data acquisition via associated
hardware and software (instrumentation). Continuous monitoring of
unrestrained animals, as opposed to older blood pressure restrained
measurement
methods. Devices measuring body temperature and biopotentials, including
electrocardiogram, may also
be used with the system. Contact:
Jesse Liu, 501-686-8106,
sliu@uams.edu
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CORE VIDEO
DNA Sequencing Facility
 The DNA Sequencing Facility uses a 3100 Genetic Analyzer (capillary technology) to provide DNA sequencing for plasmids and PCR products. A Qiagen cabinet is also present in the lab to provide select DNA and RNA isolation kits.
Contact: Allen Gies, 501-686-6927,
giesallenj@uams.edu
DNA Damage and Toxicology Facility
 The
DNA Damage and Toxicology Facility provides expertise/instrumentation to
perform DNA damage and toxicology studies related to toxic or hypoxic
tissue/cell injury in drug development, diseases or aging. In addition to
offering standard assays for cytotoxicity (e.g., TUNEL or Comet assays), the
facility helps investigators measure oxidative damage and quantify levels of
apoptosis and necrosis in cells and tissues by using quantitative
cytochemistry, immuno-cytochemistry techniques, and 3-D imaging.
Contact:
Alexei G. Basnakian, 501-352-2870,
basnakianalexeig@uams.edu
Experimental Pathology Laboratory
 The
Experimental Pathology Shared Resource provides centralized, comprehensive
histological services, with convenient access to all services within one
facility. Stringent quality control standards are maintained with GLP
compliance upon request. The laboratory director, consultants, and
technicians have extensive experience in routine histology and
immunohistochemistry involving both human and animal tissues. Services
include: processing and embedding of tissues, frozen and paraffin-embedded
tissue sections, routine staining, a wide range of special histochemical
stains, immunohistochemisrty, veterinary pathology consultation, and digital
slide scanning and analysis using the Aperio Scanscope. Contact:
Leah Hennings, 501-526-7624,
lhennings@uams.edu -
CORE VIDEO
Pharmacogenomics Facility
 The
Pharmacogenomics Facility provides gene expression including
microRNA, for human, mouse/rat samples using Bead expression chips
and BeadArray Reader. SNP chips are also performed (e.g., 1 million
human SNPs and numerous CNVs can be detected with the 1 M-duo human
SNP chip). The core has a dedicated Tecan Robot for post-processing
of Infinium SNP Chips. The Illumina Genome Analyzer is a high-end
sequencer and can be used for direct sequencing of any genome/cDNA
library. The BeadXpress Reader works with Veracode technology
resulting in high throughput and muliplex detection of SNPs, gene
expression, and protein detection from a custom design. A
QIAextractor robot performs extraction from any Qiagen kit.
Contact: Marjorie L.
Beggs, 501-296-1504 X1835, beggsmarjoriel@uams.edu
Proteomics Facility
 The
Proteomics Facility provides mass spectrometric analysis and identification of proteins.
It is equipped with a Thermo LTQ XL linear ion trap mass spectrometer with electron transfer dissociation capability, a PerkinElmer SCIEX MALDI-prOTOF mass spectrometer, a Waters Q-Tof Micro tandem mass spectrometer and a Thermo LTQ linear ion trap mass spectrometer. Additional gel band analysis equipment includes a ProPic imaging and spot-picking robot from Genomic Solutions, a ProGest in-gel enzymatic digestion robot from Genomic Solutions, and a Bio-Rad 2D gel electrophoresis system. Contact:
Sam Mackintosh, 501-526-5978,
mackintoshsamuelg@uams.edu
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CORE VIDEO
Transgenic
Facility
 The
Transgenic Facility generates transgenic mice via pronuclear microinjection
of DNA constructs supplied by users. Microinjections are performed in
embryos obtained from C57BL/6 or CB6F1 (a cross between BALB/c and C57BL/6) mice.
Personnel are also available for consultation regarding DNA construct design and animal husbandry.
Contact: Charles O’Brien, 501-686-5607,
caobrien@uams.edu, or Joseph Goellner, 501-940-7264,
jjgoellner@uams.edu -
CORE VIDEO
Microbiology & Immunology Flow Cytometry
 The Microbiology & Immunology Flow Cytometry Facilities provide researchers with an immensely powerful and diverse tool to measure multiple physical characteristics of individual cells in suspension. The flow cytometer can indicate relative cell size and density or complexity by measuring forward- and side-scattered laser light, respectively. In addition, the flow cytometer can measure relative fluorescence from fluorescent probes which bind to specific cell-associated molecules.
Contact: Andrea Fox, 501-686-5145,
afox@uams.edu
Bioluminescent and Fluorescent Imaging
Facilities
 The
Xenogen IVIS Imaging System is a highly sensitive CCD camera optimized for
biophotonic imaging (e.g. in vitro and in vivo assays using reporter cells
tagged for bioluminescence and/or fluorescence.
Contact:
Bill Hogue, 501-686-8911,
bhogue@uams.edu
Skeletal Phenotyping
Facility
 Performs
high resolution skeletal
imaging and analysis utilizing bone densitometry (DXA), perpipheral
quantitated computed tomography (pQCT) or micro CT (uCT). Skeletons can be
analyzed in vivo or ex vivo. When combined with histological evaluation,
detailed insight into skeletal phenotype is obtained.
Contact:
Larry J. Suva, 501-526-6110,
suvalarryj@uams.edu
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CORE VIDEO
Digital Microscopy Laboratory
 This
facility operates
and maintains computer controlled microscope workstations capable of static
and time lapse (e.g. minutes to days) imaging of living cells. Fluorescence
imaging and multicolor images from fixed and living samples are generated.
Microscopes are inverted and designed for analysis of living cells. However,
fixed samples may be studied as well.
Contact:
Brian Storrie, 501-526-7418,
storriebrian@uams.edu -
CORE VIDEO
Radiological
Imaging Facility
 The
MicroPET Facility consists of a Siemens FOCUS MicroPET that can produce
whole body or regional PET images of animals ranging in size from mice to
small dogs; and can image the head and appendages of human subjects. An
on-site cyclotron and a fully staffed Radiochemistry Laborotory provide a
wide range of PET markers for research projects involving PET imaging.
Custom PET Markers can also be developed in collaboration with the
facility's staff.
Contact: Director: Michael J. Borrelli, 501-526-5897,
mjborelli@uams.edu
Manager:
Terri L. Alpe, 501-590-0457,
alpeterril@uams.edu
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CORE VIDEO
Tissue
Procurement Facility
The Tissue Procurement Facility is a campus-wide shared research core that
coordinates/oversees the donation, processing, storage, and dissemination
of tissue and cell suspension specimens and related data for all UAMS researchers. Our inventory
includes human tissues, serum, and bone marrow mononuclear cell extracts,
with plans to bank urine and other fluid products. Our database is managed
using caTissue, an online-accessible biorepository software. caTissue is
part of caBIG®, an NIH-sponsored information network enabling the cancer
community to share data and knowledge. Our database can be searched using
pathological and clinical parameters. Contact:
Tissue Procurement Facility or Rémelle Eggerson, 501-296-1504
x1705,
rmeggerson@uams.edu. |
