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Wayne P. Wahls, Ph.D.
Professor
Ph.D., University of Illinois, Chicago
Chromosome Dynamics in
Meiosis; Combinatorial bZIP
Dimers and Cellular Growth Control
During meiosis, homologous chromosomes replicate once, pair,
enjoy a high rate of recombination, and undergo two rounds of chromosome
segregation to produce haploid meiotic products. We use a combination of genetic, molecular, biochemical, and
cytological approaches to study meiotic chromosome dynamics
in fission yeast.
One focus is on how recombination hotspots and chromatin remodeling regulate
recombination throughout the genome. We are also investigating how complexes of
meiotic recombination enzymes assemble and function. A third focus is on the
relationship between recombination, sister chromatid cohesion, and proper
segregation of chromosomes in each meiotic division.
Proteins of the ATF/CREB/AP-1 family are components of signal transduction
pathways that monitor intracellular and extracellular conditions and transmit
those signals to downstream targets. These proteins share a conserved bZIP
domain that mediates both protein dimerization and sequence-specific DNA binding
activity. A systematic study of bZIP transcription factors of fission yeast is
underway. We are interested in how different dimers form, what sets of target
genes they activate in response to different cellular stresses, and how upstream
signal transduction events regulate the process.
Selected Publications
Kan, F., M.K.
Davidson, and W.P. Wahls (2011). Meiotic recombination protein Rec12:
functional conservation, crossover homeostasis and early crossover/non-crossover
decision. Nucleic Acids Res. 39:1460-1472. [Abstract]
Wahls, W.P., and M.K. Davidson (2010). Discrete DNA sites regulate global
distribution of meiotic recombination. Trends Genet. 26:202-208. [Abstract]
Gao, J., M.K. Davidson, and W.P. Wahls (2009). Phosphorylation-independent
regulation of Atf1-promoted meiotic recombination by stress-activated, p38
kinase Spc1 of fission yeast. PLoS ONE 4:e5533. [Abstract]
Wahls, W.P., E.R. Seigel, and M.K. Davidson (2008). Meiotic recombination
hotspots are directed to loci that express non-coding RNA. PLoS ONE
3:e2887. [Abstract]
Wahls, W.P., and M.K. Davidson (2008). Low-copy episomal vector pFY20 and
high-saturation coverage genomic libraries for the fission yeast
Schizosaccharomyces pombe (2008). Yeast 25:643-650. [Abstract]
Gao, J., M.K. Davidson, and W.P. Wahls (2008). Distinct regions of ATF/CREB
proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic
stress response. Nucleic Acids Res. 36:2838-2851. [Abstract]
DeWall, K.M., M.K. Davidson, and W.P. Wahls (2005). A DNA binding motif
of meiotic recombinase Rec12 (Spo11) defined by essential glycine-202, and
persistence of Rec12 protein after completion of meiotic recombination. Gene
356:77-84. [Abstract]
Wu, H., J. Gao, W.D. Sharif, M.K. Davidson, and W.P. Wahls (2004).
Purification, folding, and characterization of Rec12 (Spo11) meiotic recombinase
of fission yeast. Protein Expr. Purif. 38:136-144. [Abstract]
Davidson, M.K., H.K. Shandilya, K. Hirota, K. Ohta, and W.P. Wahls
(2004). Atf1-Pcr1-M26 complex links stress-activated MAPK and cAMP-dependent
protein kinase pathways via chromatin remodeling of cgs2+. Nucleic Acids Res.
279:50857-50863. [Abstract]
Davidson, M.K., N.P. Young, G.G. Glick, and W.P. Wahls (2004). Meiotic
chromosome segregation mutants identified by insertional mutagenesis of fission
yeast Schizosaccharomyces pombe: tandem-repeat, single site integrations.
Nucleic Acids Res. 32:4400-4410. [Abstract]
Yamada, T., K. Mizuno, K. Hirota, N. Kon, W.P. Wahls, E. Hartsuiker, H.
Murofushi, T. Shibata, and K. Ohta (2004). Roles of histone acetylation and
chromatin remodeling factor in a meiotic recombination hotspot. EMBO J.
23:1792-1803. [Abstract]
PubMed link to additional publications
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E-mail: |
WahlsWayneP@UAMS.edu |
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Office: |
(501)
686-5787
Biomedical Research Center
405F |
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Lab: |
(501)
686-7876 Biomedical Research Center 403/404 |
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FAX: |
(501)
526-7008 |
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