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Wayne P. Wahls, Ph.D.
Associate Professor
Ph.D., University of Illinois, Chicago
Chromosome Dynamics in
Meiosis; Combinatorial bZIP
Dimers and Cellular Growth Control
During meiosis, homologous chromosomes replicate once, pair,
enjoy a high rate of recombination, and undergo two rounds of chromosome
segregation to produce haploid meiotic products. We use a combination of genetic, molecular, biochemical, and
cytological approaches to study meiotic chromosome dynamics
in fission yeast.
One focus is on how recombination hotspots and chromatin remodeling regulate
recombination throughout the genome. We are also investigating how complexes of
meiotic recombination enzymes assemble and function. A third focus is on the
relationship between recombination, sister chromatid cohesion, and proper
segregation of chromosomes in each meiotic division.
Proteins of the ATF/CREB/AP-1 family are components of signal transduction
pathways that monitor intracellular and extracellular conditions and transmit
those signals to downstream targets. These proteins share a conserved bZIP
domain that mediates both protein dimerization and sequence-specific DNA binding
activity. A systematic study of bZIP transcription factors of fission yeast is
underway. We are interested in how different dimers form, what sets of target
genes they activate in response to different cellular stresses, and how upstream
signal transduction events regulate the process.
Selected Publications
Sharif, W.D., G.G. Glick, M.K. Davidson, and
W.P. Wahls (2002). Distinct functions of S. pombe Rec12 (Spo11) protein and
Rec12-dependent crossover recombination (chiasmata) in meiosis I; and a
requirement for Rec12 in meiosis II. Cell Chromosome.
1:1. [Abstract]
[PDF
Reprint]
Davidson, M.K. H.K. Shandilya, K. Hirota, K. Ohta,
and W.P. Wahls (2004). Atf1-Pcr1-M26 complex links stress-activated MAPK
and cAMP-dependent protein kinase pathways via chromatin remodeling of cgs2+.
J. Biol. Chem. 279:50857-50863. [Abstract]
[PDF
Reprint]
Wu, H., J. Gao, W.D. Sharif, M.K. Davidson, and
W.P. Wahls (2004). Purification, folding, and characterization of Rec12
(Spo11) meiotic recombinase of fission yeast. Protein Expr Purif.
38:136-144. [Abstract]
[PDF
Reprint]
Davidson, M.K., N.P. Young, G.G. Glick, and W.P.
Wahls (2004). Meiotic chromosome segregation mutants identified by
insertional mutagenesis of fission yeast Schizosaccharomyces pombe;
tandem-repeat, single-site integrations. Nucleic Acids Res.
32:4400-4410. [Abstract]
[PDF
Reprint]
Yamada, T., K. Mizuno, K. Hirota, N. Kon, W.P.
Wahls, E. Hartsuiker, H. Murofushi, T. Shibata, and K. Ohta (2004). Roles
of histone acetylation and chromatin remodeling factor in a meiotic
recombination hotspot. EMBO J. 23:1792-1803.
[Abstract]
[PDF
Reprint]
DeWall, K.M., M.K. Davidson, W.D. Sharif, C.A.
Wiley, and W.P. Wahls (2005). A DNA binding motif of meiotic recombinase
Rec12 (Spo11) defined by essential glycine-202, and persistence of Rec12 protein
after completion of recombination. Gene. 356:77-84.
[Abstract]
[PDF
Reprint]
PubMed link to additional publications
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E-mail: |
WahlsWayneP@UAMS.edu |
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Office: |
(501)
686-5787
Biomedical Research Center
405F |
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Lab: |
(501)
686-7876 Biomedical Research Center 403/404 |
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FAX: |
(501)
526-7008 |
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