Alan Tackett Ph.D.

Associate Professor

Ph.D. University of Arkansas for Medical Science

My laboratory focuses on histone epigenetic mechanisms that regulate gene transcription and that are coupled to melanoma progression. We utilize a suite of techniques in our studies including proteomics of human biopsies, immunohistochemistry, cell culture, tumorigenicity assays, ChIPseq, biochemical and proteomic approaches for analyses of protein complexes, and cutting-edge mass spectrometry for the analysis of histone post-translational modifications. We have a unique environment that provides for discovery-based studies like proteomics as well as hypothesis-driven studies like those we perform in cell culture and with skin biopsies. In addition to our melanoma work, we develop new technologies for epigenetic studies such as tools for detection of in vivo protein interactions and quantitative assays for histone modifying proteins.
 

Selected Publications

Byrum, S.D., Taverna, S.D. and Tackett, A.J. (2011) Quantitative analysis of histone exchange for transcriptionally active chromatin. J Clin Bioinforma, accepted

Byrum, S., Avaritt, N.L., Mackintosh, S.G., Munkberg, J.M., Badgwell, B.D., Cheung, W.L. and Tackett, A.J. (2011) A Quantitative Proteomic Analysis of FFPE Melanoma. J Cutan Pathol, accepted

Blair, L.P., Avaritt, N.L., Huang, R., Cole, P.A., Taverna, S.D. and Tackett, A.J. (2011) MassSQUIRM: An Assay for Quantitative Measurement of Lysine Demethylase Activity. Epigenetics, 6, 490-499.

Byrum, S., Mackintosh, S.G., Edmondson, R.D., Cheung, W.L., Taverna, S.D. and Tackett, A.J. (2011) Analysis of Histone Exchange during Chromatin Purification. JIOMICS, 1, 61-65.

Smart, S.K., Mackintosh, S.G., Edmondson, R.D., Taverna, S.D. and Tackett, A.J. (2009) Mapping the local protein interactome of the NuA3 histone acetyltransferase. Protein Science, 18, 1987-1997.
 

Pubmed link to additional publications