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Alan B. Diekman, Ph.D.
Assistant Professor
Ph.D., Northwestern University
B.S., Hope College
Structure
and function of glycoconjugates in the mammalian reproductive tract
Glycoconjugates in the mammalian
reproductive tract are critical components of the molecular mechanisms that
control sperm maturation, sperm transport, and sperm-egg interactions. The dense
carbohydrate layer on the mammalian sperm surface, the glycocalyx, is
extensively modified during sperm transport and maturation. Multiple functions
have been proposed for the sperm glycocalyx and sperm-surface carbohydrates have
been implicated in immune-mediated human infertility. Our research has focused
on CD52, a low molecular weight, highly acidic, GPI-anchored glycoprotein that
is a major component of the sperm glycocalyx. CD52 is a maturation factor
acquired by spermatozoa during epididymal transport, is potentially involved in
gamete interactions during fertilization, and may serve as a target for
antibodies involved in immune response to spermatozoa. Research has focused on
both structural and functional aspects of the molecule.
Selected Publications
Westbrook, V. A., Schoppee, P. D., Vanage,
G. R., Klotz, K. L., Diekman, A. B., Flickinger, C. J.,
Coppola, M. A., Herr, J. C. (2006)
Hominoid-specific SPANXA/D genes demonstrate differential
expression in individuals and protein localization to a distinct nuclear
envelope domain during spermatid morphogenesis. Molecular
Human Reproduction In Press. [Abstract]
Westbrook, V. A.,
Schoppee, P. D., Diekman, A. B., Klotz, K. L., Allietta, M.,
Hogan, K. T., Slingluff, C. L., Patterson, J. W., Frierson, H. F., Irvin, W. P.
Jr, Flickinger, C.J., Coppola, M.A., Herr, J. C. (2004)
Genomic organization, incidence, and localization of the SPAN-x
family of cancer-testis antigens in melanoma tumors and cell lines.
Clinical Cancer Research
10:101-112. [Abstract]
Shetty, J., Wolkowicz, M. J., Digilio, L.
C., Klotz, K. L., Jayes, F. L., Diekman A. B., Westbrook, V.
A., Farris, E. M., Hao, Z., Coonrod, S. A., Flickinger, C. J., Herr, J. C. (2003)
SAMP14, a
novel, acrosomal membrane-associated, glycosylphosphatidylinositol-anchored
member of the Ly-6/urokinase-type plasminogen activator receptor superfamily
with a role in sperm-egg interaction. Journal of Biological
Chemistry 278:30506-15 [Abstract]
Diekman, A. B. (2003)
Glycoconjugates in Sperm Transport and Gamete Interactions: How Much Sugar Does
It Take to Sweet Talk the Egg? Cellular and Molecular Life Sciences
60:298-308.
[Abstract]
Norton, E. J., Diekman, A. B., Westbrook, V. A., Klotz,
K. L., Thomas, T. S., Wright, D. C., Brisker, J., Flickinger, C. J., Herr, J. C.
(2003) A male genital tract-specific carbohydrate epitope on human CD52:
Implications for immunocontraception. Tissue Antigens 60:354-364.
[Abstract]
Mandal, A., Klotz, K. L., Shetty, J., Jayes, F. L., Wolkowicz,
M. J., Bolling, L. C., Coonrod, S. A., Black, M. B., Diekman, A. B.,
Haystead, T. A. J., Flickinger, C. J., Herr, J. C. (2003) SLLP1, a unique,
intra-acrosomal, non-bactriolytic, c lysozyme-like protein of human spermatozoa.
Biology of Reproduction 68:1525-1537.
[Abstract]
Visconti, P. E., Westbrook, V. A., Chertihin, O., Demarco, I.,
Sleight, S., Diekman, A. B. (2002) Novel signaling pathways involved in
sperm acquisition of fertilizing capacity. Journal of Reproductive Immunology
53:133-150.
[Abstract]
McCauley, T. C., Kurth, B. E., Norton, E. J., Klotz, K. L.,
Westbrook, V. A., Rao, A. J., Herr, J. C., Diekman, A. B. (2002) Analysis
of a human sperm CD52 glycoform in primates: Identification of an animal model
for immunocontraceptive vaccine development. Biology of Reproduction 66:1681-1688.
[Abstract]
Westbrook, V. A., Diekman, A. B., Herr, J. C., Visconti,
P. E. (2002) Novel signaling pathways involved in sperm acquisition of
fertilizing capacity. In: Introduction to Mammalian Reproduction. (Ed. D.
R. Tulsiani). Kluwer Academic/Plenum Publishers, New York, NY.
Shetty, J., Diekman, A. B., Jayes, F., Sherman, N.,
Naaby-Hansen, S., Flickinger, C. J., Herr, J. C. (2001) Differential extraction
and enrichment of human sperm surface proteins in a proteome: Identification of
immunocontraceptive candidates. Electrophoresis 22:3053-3066.
[Abstract]
Norton, E. J., Diekman, A. B., Westbrook, V. A.,
Flickinger, C. J., Herr, J. C. (2001) RASA, an anti-sperm ScFv directed against
the sperm glycoform of CD52: Implications for novel contraceptives. Human
Reproduction 16:1854-1860. [Abstract]
Westbrook, V. A., Diekman, A. B., Naaby-Hansen, S.,
Coonrod, S. L., Klotz, K. L., Thomas, T. S., Norton, E. J., Flickinger, C. J.,
Herr, J. C. (2001) Differential nuclear localization of the cancer-testis
associated protein SPAN-X/CTp11 in transfected cells and in 50% of human
spermatozoa. Biology of Reproduction 64, 345-358.
[Abstract]
Diekman, A. B., Norton, E. J.,
Westbrook, V. A., Klotz, K. L., Naaby-Hansen, S., Herr, J. C. (2000) Anti-sperm
antibodies from infertile patients and their cognate sperm antigens: A review.
Identity between SAGA-1, the H6-3C4 antigen, and CD52. American Journal of
Reproductive Immunology 43:134-143.
[Abstract]
Westbrook, V. A., Diekman, A. B.,
Klotz, K. L., Khole, V. V. von Kap-Herr, C., Golden, W. L., Eddy, R. L., Shows,
T. B., Stoler, M. H., Lee, C. G., Charles J. Flickinger, C. J., Herr, J. C.
(2000) Spermatid-specific expression of the novel X-linked gene product SPAN-X
localized to the nucleus of human spermatozoa. Biology of Reproduction
63:469-481.
Diekman, A. B., Norton, E. J.,
Klotz, K. L., Westbrook-Case, V. A., Shibahara, H., Naaby-Hansen, S., Flickinger,
C. J., Herr, J. C. (1999) N-linked glycan of a sperm CD52 glycoform associated
with human infertility. The FASEB Journal 13:1303-1313.
Diekman, A. B., Norton, E. J.,
Klotz, K. L., Westbrook-Case, V. A., Herr, J.C. (1999) Evidence for a unique
N-linked glycan associated with human infertility on sperm CD52: A Candidate
Contraceptive Vaccinogen. Immunological Reviews 171:203-211.
Diekman, A. B., Olson, G.,
Goldberg, E. (1998) Expression of the human antigen SPAG2 in the testis and
localization to the outer dense fibers in spermatozoa. Molecular Reproduction
and Development 50:284-293.
Diekman, A. B., Westbrook-Case,
V. A., Klotz, K. L., Naaby-Hansen, S., Flickinger, C.J., Herr, J. C. (1997)
Biochemical characterization of Sperm Agglutination Antigen-1, a human sperm
surface antigen implicated in gamete interactions. Biology of Reproduction
57:1136-1144.
Diekman, A. B.,
Herr, J. C. (1997) Sperm antigens and their use in the development of an
immunocontraceptive. American Journal of Reproductive Immunology 37:111-117.
Diekman, A. B., Goldberg, E.
(1995) Sperm Antigens: What are their private specificities? In: Immunology
of Reproduction (Eds. M. Kurpisz and N. Fernandez). BIOS Scientific
Publishers, Oxford, United Kingdom, p. 1-21.
Herr, J. C., Diekman, A. B., Coonrod, S. A., Freemerman,
A. J. (1995) Recent advances in the path to a contraceptive vaccine. In:
Proceedings of the Fifth World Congress on Fertility and Sterility. (Eds. B.
Hedon, J. Bringer and P. Mares). Parthenon Press, Carnforth, United Kingdom, p.
35-46.
Diekman, A. B., Goldberg, E.
(1994) Characterization of a human antigen with sera from infertile
patients. Biology of Reproduction 50:1087-1091.
Member of the American Society of Andrology. The Society
fosters a multi-disciplinary approach to the study of male reproduction and
exists to promote scientific interchange and knowledge of the male reproductive
system.
http://www.andrologysociety.com
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E-mail: |
DiekmanAlan@uams.edu |
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Office: |
(501) 686-5812 Biomedical
Research Center 421E |
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Lab: |
(501) 686-5195 Biomedical
Research Center 419 |
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FAX: |
(501) 686-8169 |
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