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Alan B. Diekman, Ph.D.
Associate Professor
Ph.D., Northwestern University
B.S., Hope College
Lectins and glycoconjugates in the mammalian reproductive tract
Glycoconjugates in the mammalian
reproductive tract are critical components of the molecular mechanisms that
control sperm maturation, sperm transport, and sperm-egg interactions. The dense
carbohydrate layer on the mammalian sperm surface, the glycocalyx, is
extensively modified during sperm transport and maturation. Multiple functions
have been proposed for the sperm glycocalyx and sperm-surface carbohydrates have
been implicated in immune-mediated human infertility. Our research has focused
on CD52, a low molecular weight, highly acidic, GPI-anchored glycoprotein that
is a major component of the sperm glycocalyx. CD52 is a maturation factor
acquired by spermatozoa during epididymal transport, is potentially involved in
gamete interactions during fertilization, and may serve as a target for
antibodies involved in immune response to spermatozoa. Research has focused on
both structural and functional aspects of the molecule.
Selected Publications
Saraswati, S. Block, A. S., Davidson, M. K.,
Rank, R. G., Mahadevan, M., Diekman, A. B. (2010) Galectin-3 is a
substrate for prostate-specific antigen in human seminal plasma. The Prostate
DOI: 10.1002/pros.21236.
Block, A. S., Saraswati, S. Lichti, C. F.,
Mahadevan, M., Diekman, A. B. (2010) Co-purification of Mac-2 binding
protein with galectin-3 and association with prostasomes in human semen. The
Prostate In Press.
Saraswati, S., Block, A. S.,
Diekman, A.
B.
(2010) Galectins and theirs role in various biological processes. In: Carbohydrate
Recognition: Biological Problems, Methods, and Applications (Eds. Binghe
Wang and Geert-Jan Boons). John Wiley & Sons, Inc.
In Press.
Naaby-Hansen, S., Diekman,
A., Shetty, J., Flickinger, C. J., Westbrook, A., Herr, J. C. Identification
of calcium-binding proteins associated with the human sperm plasma membrane
(2010) Reproductive Biology and Endocrinology, 8:6
DOI 10.1186/1477-7827-8-6 [Abstract]
Jones, J. L., Saraswati S., Block A. S., Lichti C. F., Mahadevan
M., Diekman A. B. (2010) Galectin-3 is associated with prostasomes in
human semen. Glycoconjugate Journal 27:227-36 DOI
10.1007/s10719-009-9262-9 [Abstract]
Tyler, C., Lichti, C.,
Diekman, A.B., Foley, S. (2008) Evaluation of differentially expressed
proteins following complement exposure in avian pathogenic Escherichia coli.
Avian Diseases 52:23-27 [Abstract].
Parry, S., Wong, N.K., Easton, R.L., Panico, M.,
Haslam, S.M., Morris, H.R., Anderson, P., Klotz, K.L., Herr, J.C., Diekman,
A.B., Dell, A.
(2007) The sperm agglutination antigen-1 (SAGA-1) glycoforms of CD52 are O-glycosylated.
Glycobiology
17:1120- 126 [Abstract].
Westbrook, V. A.,
Schoppee, P. D., Diekman, A. B., Klotz, K. L., Allietta, M.,
Hogan, K. T., Slingluff, C. L., Patterson, J. W., Frierson, H. F., Irvin, W. P.
Jr, Flickinger, C.J., Coppola, M.A., Herr, J. C. (2004)
Genomic organization, incidence, and localization of the SPAN-x
family of cancer-testis antigens in melanoma tumors and cell lines.
Clinical Cancer Research
10:101-112. [Abstract]
Diekman, A. B. (2003)
Glycoconjugates in Sperm Transport and Gamete Interactions: How Much Sugar Does
It Take to Sweet Talk the Egg? Cellular and Molecular Life Sciences
60:298-308.
[Abstract]
Norton, E. J., Diekman, A. B., Westbrook, V. A., Klotz,
K. L., Thomas, T. S., Wright, D. C., Brisker, J., Flickinger, C. J., Herr, J. C.
(2003) A male genital tract-specific carbohydrate epitope on human CD52:
Implications for immunocontraception. Tissue Antigens 60:354-364.
[Abstract]
Visconti, P. E., Westbrook, V. A., Chertihin, O., Demarco, I.,
Sleight, S., Diekman, A. B. (2002) Novel signaling pathways involved in
sperm acquisition of fertilizing capacity. Journal of Reproductive Immunology
53:133-150.
[Abstract]
McCauley, T. C., Kurth, B. E., Norton, E. J., Klotz, K. L.,
Westbrook, V. A., Rao, A. J., Herr, J. C., Diekman, A. B. (2002) Analysis
of a human sperm CD52 glycoform in primates: Identification of an animal model
for immunocontraceptive vaccine development. Biology of Reproduction 66:1681-1688.
[Abstract]
Shetty, J., Diekman, A. B., Jayes, F., Sherman, N.,
Naaby-Hansen, S., Flickinger, C. J., Herr, J. C. (2001) Differential extraction
and enrichment of human sperm surface proteins in a proteome: Identification of
immunocontraceptive candidates. Electrophoresis 22:3053-3066.
[Abstract]
Diekman, A. B., Norton, E. J.,
Klotz, K. L., Westbrook-Case, V. A., Shibahara, H., Naaby-Hansen, S., Flickinger,
C. J., Herr, J. C. (1999) N-linked glycan of a sperm CD52 glycoform associated
with human infertility. The FASEB Journal 13:1303-1313.
Member of the American Society of Andrology. The Society
fosters a multi-disciplinary approach to the study of male reproduction and
exists to promote scientific interchange and knowledge of the male reproductive
system.
http://www.andrologysociety.com
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E-mail: |
DiekmanAlan@uams.edu |
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Office: |
(501) 686-5812 Biomedical Research Center 421B |
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Lab: |
(501) 686-5195 Biomedical
Research Center 419 |
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FAX: |
(501) 686-8169 |
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